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KMID : 0380020040190060446
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Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 6 p.446 ~ p.450
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Culture Conditions for Hydrogen Production of Enterobacter cloacae YJ-1
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Lee Ki-Seok
Kang Chang-Min
Jung Sun-Yong
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Abstract
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We investigated the effective culture conditions of anaerobic bacteria, Enterobacter cloacae YJ-1 on hydrogen production. It was cultured with 60 mL of working volume at , 120 rpm for 40 h. With culture time, hydrogen production and cell growth increased, but residual glucose and pH decreased. When the of glucose was used as single carbon source, hydrogen production was 975.1 mL/L. To enhance hydrogen productivity, mixed carbon sources of glucose and sucrose were added. The maximum hydrogen production was earned at the mixing ratio of 25:75, and it was 1319.5 mL/L. When we added 50 mM of phosphate to protect the pH drop in culture broth, hydrogen production increased 1.3 times more than that of initial concentration. The organic nitrogen sources were more effective than inorganic nitrogen for hydrogen production. Among organic nitrogen, yeast extract was the most effective and its hydrogen production was 1691.3 mL/L. Among 9 of mineral sources, Ferric citrate and were especially effective, and their productions were 1782.3 mL/L and 1784.8 mL/L, respectively
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KEYWORD
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Hydrogen production, organic acid, inhibition, mixed carbon sources
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